Frozen sexed semen needs to be thawed using temperature regulated thawing units and utilizing a thermometer to monitor temperature. Water temperature should be between 35°C - 37°C. Pick the straw with a forceps out of the Nitrogen, shake off and put quickly in the water. The thawing process should be at least 45 seconds and no more than 15 minutes.
Frozen sexed semen is packaged in ‘.4 cc straws; it must be used with ‘/4 cc guns. Warm-up the gun by tight rubbing and/or wear the gun between the clothing. Take the straw out of the water, dry and put in the gun. Cut the straw ca. 1 cm from top with a straight out and ﬁnalize the gun with the syringe.
Keep the gun warm between your clothes, close to your body
Keep the interval between thawing process and insemination short; 5 minutes is recommended.
it is recommended to only use sexed semen in virgin heifers or in cows with an excellent record of conception through Al.
It is recommended to Al 12 hours after the first signs of standing heat. Use in animals that show a clear sign of good heat.
Avoid using sexed semen in animals that have had more than two or three services.
Use sexed semen in well established AI programs and preferably by experienced Al technicians.
The herd needs to have very good nutritional, sanitary and reproductive programs.
Avoid using sexed semen in animals that are under any kind of stressful situation that might cause problems.
During thawing the straw must pass quickly through the freezing point. This is the reason to have an increased temperature.
Keep the straw after thawing on a constant temperature. By carrying the gun close to your body, the temperature wilt be close to 35-37°C, which is also the temperature of inside the cow.